In vitro evaluation of salinity tolerance of potato (Solanum tuberosum var. Spunta)
Keywords:
Potato, in vitro, single node, NaCl stress, growth responseAbstract
In vitro evaluation of salinity (NaCI) effects on potato (Solanum tuberosum L) var. Spunta was investigated with five NaCI levels (0, 20, 40, 60, 80, and100 mM) by using single node explants. Significant differences affects were noticed among the plantlets cultivated in different NaCl levels. Salinity stress gradually depressed plantlets growth and development with increased NaCl concentration in MS media. Growth traits tested of plantlets grown in MS containing 20 mM NaCl exhibited no differences compared to control except plantlet length which was reduced significantly by 36% compared to control. Increasing salinity more than 20 mM showed a progressive reduction in all traits tested. All plantlets survived at high NaCl (100 mM) containing MS media and severely reduced growth rate. Our results estimated the moderate salinity tolerance of variety Spunta.
Downloads
References
[1.] Aghaei, K., Ehsanpour, A., Balali, G., and Mostajeran, A. 2008. In vitro screening of potato (Solanum tuberosum L.) cultivars for salt stress using physiological parameter and RAPD analysis. Am. Eurasian J. Agri. Environ Sci., 3(2): 159- 164.
[2.] Bilski, J., Nelson, D., and Conlon, R. 1988. The response of four potato cultivars to chloride salinity, sulphate salinity and calcium in pot experiments. Am. Potato J. 65: 85-90.
[3.] Bouaziz, E. 1980. Tolerance a la salure de la pomme de terre. Physio. Vege. 162 18:11-17.
[4.] Chapin, E. 1991. Integrated responses of plants to stress. A centralized system of physiological responses. Bioscience. 40: 29-31.
[5.] Daneshmand, F., Arvin, M. and Kalantari, K. 2012. Physiological responses to NaCl stress in three wild species of potato In vitro. Acta. Physiol. Plant., 32(1): 91- 101.
[6.] Dracup, M. 1991. Increasing salt tolerance of plants through cell culture requires greater understanding of tolerance mechanisms. Aust. J. Plant Physiol. 18: 1-15.
[7.] FAO. 2013. Year book of aquaculture department. Food and agriculture organization of the united nations, Rome- Italy.
[8.] Heuer, B., and Nadler, A. 1995. Growth and development of potatoes under salinity and water deficit. Aust. J Agri. Res. 46:1477-1486.
[9.] Homayoun, H., Parisa, M., and Daliri, M. 2011. Study of salinity stress effect on two potato (Solanum tuberosum L.) cultivars In vitro. Am. Eurasian J. Agri. Environ. Sci.,11 (5): 729- 732.
[10.] Khenifi, L., Boudjeniba, M., and Kameli, A. 2011. Effects of salt stress on micropropagation of potato (Solanum tuberosum L.). Afr. J. Biotechnol., 10 (40):7840-7845.
[11.] Khrais, T., Leclers, Y., and Donnelly, D. 1998. Relative salinity tolerance of potato cultivars assessed by in Vitro screening, Am. J. Potato. Res, 75: 207-210.
[12.] Maas, V., and Hoffman, J. 1977. Crop salt tolerance - current assessment. Journal of the Irrigation and Drainage Division. Proc. Am. Soc. Civil Engr. 103: 115-134.
[13.] Morpurgo, R. 1991. Correlation between Potato Clones Grown In vivo and In vitro under Sodium Chloride Stress Conditions. Plant Breed., 107: 80- 82.
[14.] Murashige, T., and Skoog, F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15: 473-479.
[15.] Murshed, R., Najla, S., Albiski, F., Kassem, I., Jbour, M., and Al-Said, H. 2015. Using growth parameters for In-vitro screening of potato varieties tolerant to salt stress. J. Agr. Sci. Tech. Vol. 17: 483-494.
[16.] Levy, D. 1992. The response of potatoes (Solanum tuberosum L.) to salinity: plant growth and tuber yields in the arid desert of Israel. Ann. Appl. Biol., 120: 547- 555.
[17.] Levy, D., Fogelman, E., and Itzhak, Y. 1988. The effect of water salinity on potatoes (Solanurn tuberosum L.): Physiological indices and yielding capacity. Pot. Res. 31: 601-610.
[18.] Naik, S., and Widholm, M. 1993. Comparison of tissue culture and whole plant responses to salinity in potato. Plant Cell, Tiss. Org. Cult. 33: 273-280.
[19.] Rahman, H., Islam, R., Hossain, M., and Haider, S. 2008. Differential Response of Potato under Sodium Chloride Stress Conditions. In vitro. J. Biol. Sci., 16: 79- 83.
[20.] Romero-Aranda, R., and Syvertsen, P. 1996. The influence of foliar applied urea nitrogen and saline solutions on net gas exchange of Citrus leaves. J. Amer. Soc. Hort. Sci. 121: 501-506.
[21.] Tal, M. 1994. In vitro selection for salt tolerance in crop plants: Theoretical and practical considerations. In vitro Cell Develop. Biolo. 30: 175-180.
[22.] Zhang, Y., and Donnelly, D. 1997. In vitro Bioassays for Salinity Tolerance Screening of Potato. Potato Res., 40: 285– 295.
