Survey and Identify of potato viruses in the western region of Libya
Keywords:
Spring and autumn planting, potato, virus, LibyaAbstract
A survey was conducted to detect viruses infecting potato crop in the spring and the autumn seasons during 2009 and 2010 in the western region of Libya. 787 samples were collected from 32 sites in six regions. All samples were identified through double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for several viruses (Potato virus Y) (PVY), (Potato virus X) (PVX), Potato virus S(PVS), (Potato virus M) (PVM) and (Potato virus A) (PVA). 221 sample were Interacted positively with potato virus Y in 2009 season with Infection rate 18.64% and 73.14% in spring and autumn seasons respectively, while two samples were reacted positively in spring with potato virus X. 75 samples were reacted positively with potato virus Y in the 2010 season at a rate of 13.3% and 36% in spring and autumn seasons respectively. The study showed that no infection of viruses PVA, PVM, PVS in both seasons. The infections were higher in 2009 than 2010.Four tobacco plant species were used as indicator plants to study the symptoms of PVY. Mosaic, leaf malformation, dwarf and vein clearing were the main varied symptoms that’s showed in this study. This is the first report of Potato Virus Y (PVY) on potato crop in Libya
Downloads
References
[1] السنوسي، عمر، محمد شقرون وجبر خليل. 1991. عزل وتعريف فيروس البطاطس Y من نبات الفلفل في ليبيا. مجلة وقاية النبات العربية 9: 49 -51.
[2] الفلاح، احمد، الصديق القريو ونور الدين القمودي. 1999. تقييم إنتاجية بعض أصناف البطاطس تحت الظروف المحلية بالجماهيرية. مجلة البحوث الزراعية 63:3-74.
[3] القمودي، هنية، جبر خليل، الطاهر أبوحليقة وخالد الدجدج. 2009. الكشف على فيرويد الدرنة المغزلية على البطاطس/ البطاطا في ليبيا. المؤتمر العاشر لعلوم وقاية النبات. بيروت، لبنان. (مستخلص) مجلد 27.
[4] عيسى، إبراهيم وهلال أحمد. 2000. الاتجاهات الحديثة في دراسة آفات محاصيل الخضر والزينة الخشبية ومكافحتها في العالم العربي. الجزء الثاني، دار الكتاب، مصر،363 صفحة.
[5] منصور، عقل، أمين عامر حاج قاسم، نداء سالم، إيليا الشويري، يوسف أبوجوده، جبر خليل ونبيل عزيز. 2008. الفيروسات التي تصيب محصول البطاطا/ البطاطس. الأمراض الفيروسية للمحاصيل الزراعية المهمة في المنطقة العربية (مكوك، خالد محي الدين، جابر فجلة، صفاء قمري)، الطبعة الأولى. الجمعية العربية لوقاية النبات، بيروت، لبنان.273:308.
[6] Beukema, H.P. 1979. Potato improvement, some factors and facts. International Agricultural Centre. Wageningen, The Netherland, 224 pp
[7] Clark, M. and A.N. Adams. 1977. Characteristics of the microplate method Enzyme – Linked Immunosorbent Assay for the detection of plant viruses. Journal General Virology, 34: 475-483.
[8] Depta, A., H.0. Przybyś and G, Korbecka. 2014. Development of Potato virus Y (PVY) infection in susceptible and resistant tobacco cultivars. Polish Journal of Agronomy. 18: 3–6.
[9] EL-Gammudi,K.A,. J.A.Khalil and M.A.Shagrun. 1992. Isolation and Identification of Potato Virus X from Potatos at Ain-Zara, Libya. The Libyan Journal of Agriculture.13:171-178.
[10] FAO Statistic yearbook 2011.
[11] Jeffries, C.J. 1998. Potato. FAO/IPGRI Technical Guidelines for the Safe Movement of Germplasm, No. 19, 177 pp. FAO & IPGRI, Rome.
[12] Ragsdale, D.W., E.B Radcliffe. E. B, DiFonzo. 2001. Epidemiology and Field Control of PVY and PLRV. Pages 237-270. In: Virus and Virus-like Diseases of Potatoes and Production of Seed-Potatoes. G. Loebenstein, G., Berger, P.H., Brunt, A.A., Lawson, R.H. (Eds.) Dordreecht, the Netherland: Kluwer Academic Publishers.
[13] Salazar, L. F. 1982. Virus Detection in Potato Seed Production. Technical information Bulletin center, Lima, Peru. 14pp.
[14] Salaraz, L.F.1996. Potato Viruses and their Control. Lima, Peru, International potato Center. 214 pp.
[15] Shukla, D. D., C.W Ward and A.A Brunt. 1994. The Potyviridae. Wallingford, UK: CAB International.
[16] Struik, P.C. & Wiersema, S.G. 1999. Seed potato technology. Wageningen, the Netherlands: Wageningen Pers. Wageningen the Netherlands.383pp
[17] Tsakiridis, J.P.and G.V.J. Gooding. 1972. Tomato spotted wilt virus in Greece. Phytopathol. Mediterranea, 11: 42-47.
[18] Jayasinghe,U . & C. Chuquillanqui. 1989. Use of indicator plants for detection of potato viruses, CIP Research Guide file:///C:/Users/pc/Desktop/pvy/PNABD864.pdf
[19] Phillip, N., M. Juan, L. Jonathan. 2009. Potato Virus Y Management for the Seed Potato Producer. University of Idaho.